Broad Institute

The Broad Institute of MIT & Harvard, a world leader in genetic research, is looking for exceptional candidates to join the Cardiovascular Disease Initiative (CVDi). The successful candidate will join a multidisciplinary team to assist in the investigation of cardiovascular disease using genetics, cell biology, biochemistry, cell lines, and model systems. The current projects will leverage recent genetic discoveries to validate potential new targets for cardiovascular diseases under the direction of Patrick Ellinor. The candidate will be involved in day-to-day laboratory work working with postdoctoral fellows and research scientists, assisting with experimental design, analyzing and troubleshooting experiments, and communicating scientific results to the Broad CVDi team.

Key Responsibilities:
- Maintain/organize cell lines and cell banks, and isolate primary cells for use in cell-based assays
- Develop and execute cell-based assays to support target validation and hit identification & optimization
- Oversee the maintenance of mouse colony and genotyping
- Assist in mouse characterization experiments (e.g. echocardiography, tissue dissection)
-Design and execute research plans to ensure that critical project requirements, timelines and deliverables are met
- Troubleshoot scientific problems with minimal guidance
- Analyze experimental data and communicate scientific results to team members
- Prepare, order, and maintain stocks of necessary reagents and supplies; organize laboratory storage spaces
- Attend project meetings to present results and coordinate with other scientists to support project goals

Requirements:
- Bachelor's degree with 2+ years or MS degree with 1+ years of relevant laboratory experience
- Experience with molecular biology (cloning, PCR, RT-PCR, qPCR, CRISPR/Cas-9 technology)
- Experience with rodent handling and colony maintenance
- Experience with in vivo drug administration and blood sampling are a plus
- Experience with tissue collection, processing, embedding, and sectioning. Histology, immunohistochemistry, and microscopy techniques are highly desired.
- Experience with mammalian cell culture using both cell lines and primary cells
- Experience with immunocytochemistry and confocal imaging.
- Experience with stem cells, iPSC-derived cardiomyocytes, and 3D cell cultures are preferred.
- Experience with siRNA transfection and viral transduction is preferred.
- Experience with biochemistry (protein purification, enzymatic assays, western blot, ELISA) is a plus
- Experience in the cardiovascular space is highly preferred.
- Ability to critically read, interpret, and adapt scientific literature
- Strong communication and presentation skills (verbal and written)
- Strong organization, documentation and time management skills with the ability to balance multiple objectives, and deliver on timelines.
- Ability to build excellent working relationships and foster collaboration in a team environment